Generator
Part:BBa_K516222:Experience
Designed by: Federica Sampietro, Susanna Zucca, Daniele Sartori Group: iGEM11_UNIPV-Pavia (2011-09-08)
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K516222
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UNIPV-Pavia iGEM 2011 |
NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.
Characterized with:
The four measurement parts pTet-RBSx-AiiA-TT were assayed by BBa_T9002 biosensor - see experience page. No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0. A significant HSL degradation was observed in E. coli TOP10 in high copy number plasmid pSB1A2. In this case, a significant difference in degradation between pTet-RBSx-AiiA-TT and the negative control was observed, also just after 7 hours. That was the proof of the good functioning (in HIGH COPY) of the enzyme. |
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